課程資訊

Clinical Serology and Immunology Laboratory 2012

TIME:                                                                     1:10-4:00 PM, Mon, Tue, Fri for group A

For group A

Date                 Topic

2/21(Tue)         Introduction (assign groups and cases; draw blood) 邱琦 千玳 于璇 仕偉

3/5(Mon)          SDS-PAGE千玳于璇

3/6 (Tue)          Western blot and protein concentration determination (BCA)于璇 邱琦

3/9 (Fri)           Salt out and Protein A affinity column于璇 仕偉

3/19 (Mon)       Immunodiffusion, ELISA (HBsAb), serum electrophoresis千玳仕偉

3/20 (Tue)        Antigen immunization and monoclonal antibody詠鈞 邱琦

3/23 (Fri)         Peripheral blood mononuclear cells isolation (draw blood) 仕偉千玳

4/9 (Mon)         Latex agglutination (RF & RPR), VDRL邱琦 于璇

4/10 (Tue)        Flow cytometry (FACSCalibur), PCR, Cell culture仕偉邱琦

4/13 (Fri)         Immunofluorescence assay (ANA), ANCA, DNA agarose gel邱琦仕偉千玳

4/23  (Mon)      Case discussion

4/24  (Tue)        Case discussion HLA typing and multiple myeloma

6/26  (Tue)        Final exam

TIME:                                                                     1:10-4:00 PM, Mon, Tue, Wed for group B

For group B

Date                 Topic

2/21(Tue)         Introduction (assign groups and cases; draw blood) 邱琦 千玳 于璇 仕偉SDS-PAGE 于璇 邱琦

2/22(Wed)        Western blot and protein concentration determination (BCA) 于璇 仕偉

2/27 (Mon)       Holiday

2/29 (Wed)       Salt out and Protein A affinity column 千玳于璇

3/12 (Mon)       Immunodiffusion, ELISA (HBsAb), serum electrophoresis千玳仕偉

3/13 (Tue)        Antigen immunization and monoclonal antibody詠鈞 邱琦

3/14 (Wed)       Peripheral blood mononuclear cells isolation (draw blood) 仕偉千玳

3/26  (Mon)      Latex agglutination (RF & RPR), VDRL邱琦 于璇

3/27 (Tue)        Flow cytometry (FACSCalibur), PCR, Cell culture仕偉邱琦

3/28 (Wed)       Immunofluorescence assay (ANA), ANCA, DNA agarose gel邱琦仕偉千玳

4/16  (Mon)      Case discussion

4/17  (Tue)        Case discussion HLA typing and multiple myeloma

6/26  (Tue)        Final exam